Expression systems

Comparison of T7- and Lac-Based Systems for the Periplasmic Expression of Human Granulocyte Macrophage Colony Stimulating Factor in Escherichia coli

Comparison of emotion expression, inhibitory / behavioral activation systems (BIS / BAS) and intolerance of uncertainty in delinquent and normal adolescents

Juvenile delinquency is a growing social phenomenon that occurs in various forms in society. Therefore, paying attention to this phenomenon is of great importance. In this regard, the aim of the present study was to compare emotion expression, inhibitory / behavioral activation systems and intolerance of uncertainty in delinquent and normal adolescents in Tehran. This study was comparative and ...

Plant-based expression systems for protein and antimicrobial peptide production

Molecular farming technology offers a unique advantage that almost any protein can be produced economically and safely under very controlled conditions. Besides traditional production systems, such as bacteria, yeasts, insects and mammal cell lines, plants can now be used to produce eukaryotic recombinant proteins, especially therapeutic ones. Their advantages as hosts for protein production in...

O-41: Comparison of Vitrification Systems inHuman Oocytes and Evaluation of HSP A1Aand MnSOD Gene Expression Patterns

Background: The aim of this study was to compare two vitrification systems (Cryotop and OPS) on mouse MII oocytes and evaluate its effects on expression of heat shock protein A1A and MnSOD genes. Materials and Methods: Groups of about 15 oocytes without cumulus complexes (granulose, et. al.) were collected and exposed to equilibration and vitrification solutions before loading the oocytes into ...

Utilization of Site-Specific Recombination in Biopharmaceutical Production

Mammalian expression systems, due to their capacity in post-translational modification, are preferred systems for biopharmaceutical protein production. Several recombinant protein systems have been introduced to the market, most of which are under clinical development. In spite of significant improvements such as cell line engineering, introducing novel expression methods, gene silencing and pr...

تهیه سازه‌های مناسب به‌منظور بیان ژن اینترفرون گامای انسانی در Leishmania tarentolae

 Background and Objective: The aim of the present study was to express recombinant human interferon-gamma in Leishmania tarentolae. The Leishmania expression system represents the combination of easy handling known from bacterial expression systems with the potential of a eukaryotic protein expression, folding and modification system. The trypanosomatid protozoan host Leishmania tarent...